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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,210)
Mouse Secondary Antibodies (14,620)
Rabbit Secondary Antibodies (6,299)
Donkey Secondary Antibodies (4,319)
Rat Secondary Antibodies (1,234)
Sheep Secondary Antibodies (701)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Pig Secondary Antibodies (77)
Horse Secondary Antibodies (74)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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3,541 – 3,555 of 72,136 results
3,541

Goat anti-Rat IgG Fc Secondary Antibody, DyLight 649 (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Rat
Host Species Goat
Conjugate DyLight 649
Applications Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin)
Isotype IgG
Concentration 0.5 mg/mL
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunocytochemistry/Immunofluorescence 1:50 - 1:500, Immunohistochemistry-Paraffin 1:50 - 1:500
Classification Polyclonal
Immunogen Rat IgG-Fc Fragment
Primary or Secondary Secondary
Test Specificity Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.
3,542

Invitrogen™ Goat anti-Canine IgG (H+L) Secondary Antibody

Goat Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species Canine
Host Species Goat
Conjugate Unconjugated
Applications ELISA,Western Blot
Form Liquid
Isotype IgG
Concentration >4.5 mg/mL
Antigen Canine IgG (H+L)
Regulatory Status RUO
Purification Method Affinity chromatography
Product Type Secondary Antibody
Formulation PBS with 0.05% sodium azide; pH 7.2
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
3,543

Goat anti-Human IgG Fc Secondary Antibody, DyLight 633, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Human
Host Species Goat
Conjugate DyLight 633
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Gene Accession No. O75015, O75015
Concentration LYOPH
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Human IgG, Fc fragment
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on human IgG
3,544

Goat anti-Chicken IgM Heavy Chain Secondary Antibody, Biotin, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Chicken
Host Species Goat
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration LYOPH
Antigen IgM Heavy Chain
Gene Symbols IGHM
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000
Gene Alias Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene ID (Entrez) 3507
Classification Polyclonal
Immunogen Chicken IgM u chain
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against other chicken heavy or light chain proteins.
3,545

Goat anti-Mouse Kappa Light Chain Secondary Antibody, DyLight 755, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Goat
Conjugate DyLight 755
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen Kappa Light Chain
Regulatory Status RUO
Purification Method Immunogen affinity purified
Gene Alias HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Gene ID (Entrez) 3514
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Mouse Kappa Light Chain
Primary or Secondary Secondary
3,546

Invitrogen™ F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor™ 488-FluoroNanogold

F(ab')2-Goat Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage 4°C, store in dark
Target Species Mouse
Host Species Goat
Conjugate Alexa Fluor 488-FluoroNanogold
Applications Immunohistochemistry,Immunomicroscopy
Form Liquid
Isotype IgG
Concentration 0.08 mg/mL
Antigen Mouse IgG (H+L)
Regulatory Status RUO
Purification Method Purified
Product Type Secondary Antibody
Formulation PBS with 0.1% BSA and 0.05% sodium azide; pH 7.4
Immunogen Gamma Immunoglobin.
Classification Polyclonal
Primary or Secondary Secondary
3,547

Goat anti-Rat IgG (H+L) Secondary Antibody, DyLight 550, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rat
Host Species Goat
Conjugate DyLight 550
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Rat IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins
3,548

Goat anti-Canine IgE Heavy Chain Secondary Antibody, DyLight 650, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Canine
Host Species Goat
Conjugate DyLight 650
Applications Dot Blot,ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Antigen IgE Heavy Chain
Regulatory Status RUO
Purification Method Immunogen affinity purified
Gene Alias Constant Region of Heavy Chain of IgE, Ig Epsilon Chain C Region, Ig Epsilon Chain C Region ND, IgE, Immunoglobulin Epsilon, immunoglobulin heavy constant epsilon
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Dog IgE
Primary or Secondary Secondary
3,549

Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 594, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Goat
Conjugate DyLight 594
Applications ELISA,Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Goat anti-Mouse IgG (H+L) Secondary Antibody was developed against mouse IgG-heavy and light chain.
Primary or Secondary Secondary
3,550

Goat anti-Rabbit IgA Secondary Antibody, PE, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Conjugate PE
Target Species Rabbit
Host Species Goat
Applications ELISA,Immunodiffusion
Form Purified
Isotype IgG
Antigen IgA
Regulatory Status RUO
Purification Method Affinity purified
Gene Alias Immunoglobulin A
Formulation PBS
Gene ID (Entrez) 3493
Classification Polyclonal
Immunogen Purified rabbit IgA
Primary or Secondary Secondary
3,551

Goat anti-Rat IgA Secondary Antibody, PE, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Conjugate PE
Target Species Rat
Host Species Goat
Applications ELISA,Immunofluorescence,Western Blot
Form Purified
Isotype IgG
Antigen IgA
Regulatory Status RUO
Purification Method Affinity purified
Gene Alias Immunoglobulin A
Formulation PBS
Gene ID (Entrez) 3493
Classification Polyclonal
Immunogen Rat IgA
Primary or Secondary Secondary
3,552

Goat anti-Porcine IgM Heavy Chain Secondary Antibody, PE, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Conjugate PE
Target Species Pig
Host Species Goat
Applications ELISA,Immunocytochemistry,Immunofluorescence,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Antigen IgM Heavy Chain
Regulatory Status RUO
Purification Method Immunogen affinity purified
Gene Alias Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Formulation PBS
Gene ID (Entrez) 3507
Classification Polyclonal
Immunogen Pig IgM
Primary or Secondary Secondary
3,553

Goat anti-Mouse IgM Heavy Chain Secondary Antibody, Allophycocyanin, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Goat
Conjugate APC
Applications ELISA,Immunoassay,Immunodiffusion,Immunohistochemistry,Immunoprecipitation,Western Blot
Form Purified
Isotype IgG
Research Discipline B Cell Development and Differentiation Markers, Immunology
Antigen IgM Heavy Chain
Regulatory Status RUO
Purification Method Affinity purified
Gene Alias Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene ID (Entrez) 3507
Formulation PBS
Classification Polyclonal
Immunogen Purified Mouse IgM, Mu chain
Primary or Secondary Secondary
3,554

Goat anti-Chicken IgG (H+L) Secondary Antibody, PerCP, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Chicken
Host Species Goat
Conjugate PerCP
Applications Dot Blot,ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation PBS
Classification Polyclonal
Immunogen Chicken IgG whole molecule
Primary or Secondary Secondary
3,555

Goat anti-Rat IgG2c Secondary Antibody, Biotin, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Conjugate Biotin
Target Species Rat
Host Species Goat
Applications ELISA,Flow Cytometry,Western Blot
Form Purified
Isotype IgG
Antigen IgG2c
Regulatory Status RUO
Purification Method Affinity purified
Formulation PBS
Classification Polyclonal
Immunogen Rat IgG2c
Primary or Secondary Secondary